Description

Section I: Hematology 1. Red Blood Cells and Anemia 3 2. Laboratory Tests in Bleeding Disorders 6 3. Automation in Hematology 10 4. Bone Marrow Examination 12 5. Cellular Components in Blood Film 14 6. Coagulation Cascade 16 7. Composition and Functions of Blood 18 8. Coombs’ Test (Anti-globin Test) 19 9. Erythropoiesis 21 10. Erythrocyte Sedimentation Rate (ESR) 22 11. Hemoglobin Estimation 24 12. Hemoglobin and its Variants 26 13. Hemostasis 29 14. LE Cell 31 15. Leukopoiesis 32 16. Leukemia 33 17. Osmotic Fragility Test 35 18. Packed Cell Volume (PCV) 37 19. Phlebotomy 39 20. Platelets 41 21. Red Cell Indices 43 22. Reticulocyte Count 44 23. Sickling Test 46 24. Total Leukocyte Count 47 25. White Blood Cells (WBCs) 50 Contents xvi Preparatory Manual of Pathology

Section II: Histological Techniques 26. Stains for Amyloid 55 27. Cryostat 56 28. Cytochemical Stains 58 29. Fixatives 61 30. Hematoxylin and Eosin Stain 64 31. Masson-Fontana Silver Stain 66 32. Microbial Staining 67 33. Microtomy 69 34. Classification of Pigments 71 35. Mucin Stains 73 36. Museum Techniques 76 37. Oil Red O Stain 78 38. Pap (Papanicolaou) Stain 79 39. Romanowsky Stain 81 40. Stains used for Collagen Demonstration 83 41. Tissue Processing 85

Section III: Histopathology and Cytology 42. Automation in Cytology 91 43. Automation in Histopathology 92 44. Electron Microscopy 93 45. Photography and Gross Microphotography 95 46. Grossing and Specimen Management 96 47. Management of Cytological Specimen 97 48. Sources, Types of Cytological and Histopathological Specimens 98

Section IV: Laboratory Management 49. Biomedical Waste (BMW) Management 101 50. Hospital Organization and Operations 103 51. Internal Quality Control Involving Quantitative Results 105 52. Inventories 107 53. Job Analysis 108 54. Management Review Meetings 109 55. Maintenance of Laboratory Refrigerators, Freezers and Other Equipment 110 56. Material Safety Data Sheet (MSDS) 112 57. Medical Ethics and their Role in Laboratory Medicine 113 Contents xvii 58. Principles of Laboratory Management 115 59. Quality Control 117 60. Quality Control in Cytology 119 61. Quality Assurance in Hematology 121 62. Quality Control in Histopathology 123 63. Record Keeping in Laboratory 126 64. Role of Medical Laboratory Technology in Healthcare 127 65. Setting up of a Blood Bank 128 66. Biohazardous Waste Management (Spill Management) 131 67. Standard Operating Procedure (SOP) 132 68. Statistical use in a Clinical Laboratory 134 69. Turnaround Time 135 70. Disposal of Pathological Waste 137

Section V: Clinical Pathology 71. Fluids 141 72. Semen Analysis 144 73. Sputum Examination 146 74. Stool Examination 148 75. Collection and Processing of Urine 150

Section VI: Transfusion Medicine 76. Blood Group Systems 157 77. Blood Grouping 159 78. Blood Banking 160 79. Crossmatching 162 80. Transfusion Reactions 163 81. Blood Components 165 82. Plasmapheresis 167 83. Record Keeping in Blood Bank 168 84. Antibody 169

Section VII: Management of COVID-19 Samples in Laboratory 85. Laboratory Diagnosis of COVID-19 173 86. COVID-19: Safe Handling and Processing of Samples in Laboratories 176 Index 179